nested pcr造句
例句与造句
- Mycoplasma detection methods - part 3 : nested pcr assay
支原体检测方法.第3部分:窝形pcr检验法 - Rapid detection of listeria monocytogenes in food with multiplex - nested pcr
检测食品中单增李斯特菌研究 - Study of the nested pcr detection salmonella typhi in the blood specimens
巢式聚合酶链反应快速检测血液标本中 - Definition of nested pcr
巢式pcr的定义 - By using the method of genome walking , two fragments were cloned from genome walker library after the semi - nested pcr . the result of sequencing the bigger fragment showed that this 706bp sequence contained the atg start codon
通过半巢式的基因步移法，经二轮pcr后，从genomewalker文库分离到2个片段，较大的片段测序表明该706bp序列含有编码ast的起始密码子atg ，其上游序列为启动子序列。 - It's difficult to find nested pcr in a sentence. 用nested pcr造句挺难的
- Using nested pcr and draft human genome searching , we successfully cloned the tsarg2 and mouse homeobox gene and made rudiment study on its functions . there were three parts in the study , and the following is its main experimental methods and results
本研究利用巢式pcr技术和人类基因组草图搜索法，从上述est出发，于2000年6月成功地克隆了人类tsarg2基因及小鼠同源基因srg2 ，并对其功能进行了初步研究。 - According to the amino acid sequence resulted from our previous research about tb22kda protein and the related literatures about gene sequence of allergenic protein in common buckwheat , we designed primers and got the structure gene successfully . by 3 ' - race method combined with nested pcr , the 3 ' - end nuclear acid sequence was also obtained ; in additon , for the 5 ' - end sequence , we selected a specific conserved nuclear acid sequence as the 5 ' primer and part of structure gene sequence as the 3 " primer , and till now , partial 5 ' - end sequence has been amplified as well
本研究根据先前分离纯化所得天然tb22kda蛋白经maldi - tof - ms (质谱法)测得的氨基酸序列和文献报道的过敏蛋白核苷酸序列设计引物，扩增克隆了该过敏蛋白结构基因的编码序列；根据测得的序列设计特异性引物，并利用3 ’ - race方法结合巢式pcr扩增得到基因的3 ’末端；依据同源性比较的结果选用一段保守序列为5 ’引物，并根据结构基因内部序列设计3 ’特异性引物，进一步获得了该基因5 ’端的部分序列。 - The gene cloning and sequence analysis of senv - d and senv - h isolated from china according to the published nucleotide sequences of sen viruses , specific primers were designed and synthesized . from the serum of two chinese patients with non - a - e hepatitis , one senv - d isolate named senv - d - bj1 spanning the complete coding region was amplified by semi - nested pcr , another isolate named senv - d - bj2 spanning the partial coding region ( including orf1 and orf2 ) was amplified too . from one blood donor serum , two senv - h isolates named senv - h12 - 1 and senv - h 12 - 2 spanning the complete coding region were amplified by nested pcr respectively
Sen病毒d和h亚型中国分离株的克隆及序列分析我们在前期工作的基础上，结合已发表的文章及基因序列，针对senv - d和senv - h基因组设计特异性引物，利用套式pcr技术从两例non - a - e肝炎患者血清中分段克隆得到了一个senv - d亚型分离株( senv - d - bj1 )的全部编码区基因序列，还得到了一个senv - d亚型分离株( senv - d - bj2 )的大部分编码区基因序列(包括orf1和orf2 ) ；从一例健康人血清中分段克隆得到了两个senv - h亚型分离株( senv - h12 - 1和senv - h12 - 2 )的全部编码区基因序列。 - Firstly , the eo genes of csfv - jl and csfv - ln9912 strains were amplified by rt - pcr and nested pcr and then sequenced after cloned into t easy vector . comparing the eo sequences with other strains eo by biosoftwares , dnastar5 . 0 and bioedit , the phylogenetic analyse revealed that all of the strains we have sequenced could be divided into groupl and groupii . two amino acid streches of 15 csfv strains eo , which form the rnase active site , and histidine residues essential for rnase catalysis in both ones were highly conserved . the eo protein propertys of antigen epitope , hydrophobicity , isoelectric point were also predicted by bioinformatic method
首先，采用rt - pcr和nest - pcr技术扩增了csfv - jl株和csfv - ln9912株eo基因，插入teasy载体后测序，应用dnastar5 . 0和bioedit软件将其与本实验室已测其它毒株和genebank中登录的csfv代表毒株eo基因序列进行比较，绘制了遗传进化树并预测了eo蛋白的抗原表位、疏水性、等电点等特性。 - Pbluebachisc - vp7 was identified and analped by compnding restriction endonuclease , pcr and nested pcr on the basis of the genetic sites of pbluebachisc , which was idenhfied as vp7 gene of prv hafied pbluebachisc - vp7 and barmidn - blue dna were cbeinfected insect cell sro and harvested mmbinan beculovirus 5d later identification with restriction empme and pcr showed vp7 gene has been arembwt comehy with baculovirus dna and namd a - l
纯化该重组质粒并与线性杆状病毒dnabac - n - blue共转染昆虫细胞sr9 ， 5d后收获重组病毒。重组杆状病毒dna分子的pcr及酶切鉴定表明，获得了prv - vp7基因与杆状病毒dna的重组子，命名为a - 1代病毒。 - Part 1 : identification of a novel gene , tsarg2 , and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell , and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis . to rapidly attain human novel gene full - length cdna sequence , the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe , which was significantly changed in cryptorchidism and represents a novel gene . furthermore , a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line
本研究分为三个部分，其主要实验方法及实验结果如下：第一章tsarg2基因的克隆与序列分析从已获得的在隐睾和正常睾丸对照中表达量有明显差异的est片段( be644542 )入手，设计了基因特异性引物和载体特异性引物进行巢式pcr扩增，结合人类基因组草图搜索法，从睾丸cdna文库中快速分离出人类睾丸凋亡相关基因的5末端而获得全长cdna ， genbank登录号为ay040204 ，同时应用生物信息学的方法克隆了该基因在小鼠中的同源基因， genbank登录号为af395083 。